Reliable allergen testing for cosmetics, fragrances, and household products

Fragrance allergens are central to EU cosmetic and chemical regulations. Whether they occur naturally in essential oils or are part of synthetic fragrance blends, their presence must be accurately measured and declared. Our laboratory offers advanced detection of 82 regulated fragrance allergens using GC-MS/MS technology. This service ensures your compliance with the EU Cosmetics Regulation 1223/2009, CLP, and IFRA standards, while also protecting consumer safety and brand reputation.

Why allergen testing is important

Across Europe, companies must list allergens on the labels of cosmetics and detergents if the concentrations exceed the legal thresholds. For cosmetics, this means that anything above 0.001% in leave-on products or 0.01% in rinse-off products must be listed.

In the past, only 26 allergens had to be listed, but the list has now been expanded to 82 substances. This change affects everyone in the supply chain—from raw material suppliers to brands and safety assessors—and makes reliable allergen testing more important than ever.

What We Test

The method covers the full spectrum of regulated allergens in fragrances, including well-known substances such as linalool, citral, geraniol, eugenol, limonene, coumarin, benzyl benzoate, and farnesol. These substances are often naturally present in essential oils, but are also found in synthetic blends. Because the content varies by source and batch, testing is the only way to guarantee accurate information.

✔ A complete list of all 82 allergens is available upon request.

Technology we use: GC-MS/MS

Our laboratory uses gas chromatography with tandem mass spectrometry (GC-MS/MS), the gold standard for the detection of allergenic odorants. This method separates complex odor mixtures into their individual components and identifies them with high precision.

What makes GC-MS/MS the best choice:

• Detects allergens at very low concentrations (ppm and ppb range).

• Provides quantitative results rather than simple “”present/absent”” findings.

• Works just as well for raw materials as for finished products.

In short, with GC-MS/MS, we can provide data you can trust for compliance and formulation decisions.

Applications of the 82 Allergen Test

Our allergen detection service is designed for a wide range of industries. Cosmetics brands use it to prepare their CPSR and product labels. Manufacturers of candles and diffusers rely on it for IFRA compliance and safe consumer labeling. Raw material suppliers benefit from verified data when selling essential oils and fragrance blends.

Other relevant applications include:

• Cosmetics: perfumes, lotions, hair care, skin care, makeup.

• Candles and home fragrances: provide safe allergen information.

• Detergents and cleaning products: comply with CLP and Detergents Regulation standards.

Benefits for your business

Accurate allergen testing offers more than just compliance. It provides independent verification of supplier documentation, ensures your product information is reliable, and supports smooth approval by safety assessors.

The key benefits are:

• Verified compliance with EU allergen disclosure regulations.

• Essential data for Cosmetic Product Safety Reports (CPSR).

• Support for creating IFRA compliance certificates.

• Transparent allergen declarations that build consumer trust.

How the process works

1. Choose a laboratory test
   • You purchase the lab test through our website.
2. Access to secure portal
   • After purchase, you will gain access to our secure portal where you can complete the intake.
3. Schedule laboratory test
   • Fill in all requested information about your product and upload relevant documentation.
4. Send samples
   • Send physical samples of your product to our laboratory. When you scan your lab test in the portal, the safety assessor will ask for the number of samples. This depends on your product and packaging.
5. Laboratory tests
   • We perform laboratory tests.
6. Results
   • Our safety assessor prepares the laboratory test results, uploads the report to the portal, and notifies you.

Who should use this test

This service is essential for:

• Cosmetics brands launching products in the EU/EEA.

• Suppliers of fragrance oils validate natural or synthetic blends.

• Manufacturers of candles and diffusers who require allergen sheets and IFRA compliance.

• Safety assessors and toxicologists who need verified analytical data.

Why testing makes a difference

Consider lavender essential oil. It naturally contains compounds such as linalool and linalyl acetate, but concentrations vary significantly depending on the harvest and origin. Without testing, it is impossible to know whether allergen levels exceed the prescribed thresholds.

Synthetic fragrance blends can be even more complex and often contain multiple allergens in varying proportions. GC-MS/MS testing ensures that these details are accurately measured and reported, safeguarding both compliance and consumer safety.

Beyond Testing

We also offer follow-up services to help you act on the results:

• Preparation of IFRA certificates.

• Creation of allergen declaration sheets in multiple EU languages.

• Toxicological pre-screening for formulations.

• Complete compliance packages including CPSR, PCN, UFI, and CLP support.

82 Fragrance Allergens (EU, Annex III)

Here is the complete list of the 82 allergenic fragrances included in the new expanded EU Cosmetics Regulation Annex III.

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#	INCI Name	CAS Number	Typical Source	Regulatory Status*
1	Alpha-Isomethyl Ionone	127-51-5	Synthetic violet-like fragrance	Limited allergen
2	Amyl cinnamate	122-40-7	Jasmine, synthetic	Limited allergen
3	Amylcinnamyl Alcohol	101-85-9	Jasmine, synthetic	Limited allergen
4	Anisyl Alcohol	105-13-5	Anise Oil, Synthetic	Limited Allergen
5	Benzyl Alcohol	100-51-6	Balsams, jasmine	Limited allergen
6	Benzyl benzoate	120-51-4	Balm, essential oils	Limited allergen
7	Benzyl innamate	103-41-3	Peru balsam	Limited allergen
8	Benzyl Salicylate	118-58-1	Ylang-ylang, synthetic	Limited allergen
9	Cinnamal	104-55-2	Cinnamon oil	Limited allergen
10	Cinnamon alcohol	104-54-1	Cinnamon leaves	Limited allergen
11	Citral	5392-40-5	Lemongrass, citrus oils	Limited allergen
12	Citronellol	106-22-9	Rose, geranium	Limited allergen
13	Coumarin	91-64-5	Tonka beans	Limited allergen
14	Eugenol	97-53-0	Clove oil	Limited allergen
15	Farnesol	4602-84-0	Rose oil, orange blossom	Limited allergen
16	Geraniol	106-24-1	Rose, palmarosa, citronella	Limited allergen
17	Hexyl cinnamal	101-86-0	Jasmine-like scent	Limited allergen
18	Hydroxycitronellal	107-75-5	Lily of the valley, synthetic	Limited allergen
19	Hydroxyisohexyl 3-cyclohexene carboxaldehyde (Lyral)	31906-04-4	Synthetic	Banned (still being monitored)
20	Isoeugenol	97-54-1	Ylang-ylang, clove	Limited allergen
21	Limonene	5989-27-5	Citrus peel oils	Limited allergen
22	Linalool	78-70-6	Lavender, coriander	Limited allergen
23	Methyl-2-octynoate (Methylheptinecarbonate)	111-12-6	Synthetic, fruity-green	Limited allergen
24	Evernia Prunastri extract (Oakmoss)	90028-68-5	Moss extract	Limited allergenicity
25	Evernia Furfuracea extract (Tree moss)	90028-67-4	Moss extract	Restricted allergen
26	2-(4-tert-Butylbenzyl)propionaldehyde (Lilial)	80-54-6	Synthetic flowers	Prohibited (still being monitored)
27	Allyl Heptanoate	142-19-8	Pineapple-like, synthetic	Limited allergen
28	Allyl Hexanoate	123-68-2	Pineapple, synthetic	Limited allergen
29	Allyl Phenoxyacetate	7493-74-5	Sweet floral, synthetic	Limited allergen
30	Allyl 3-cyclohexylpropionate	2705-87-5	Fruity, synthetic	Limited allergen
31	Anethole	4180-23-8	Anise, fennel	Limited allergen
32	Benzaldehyde	100-52-7	Almonds, cinnamon oil	Limited allergen
33	Benzyl acetate	140-11-4	Jasmine, ylang-ylang	Limited allergen
34	Benzyl formate	104-57-4	Fruity, balsamic	Limited allergen
35	Benzyl eugenol	120-11-6	Synthetic	Limited allergen
36	β-carophyllene	87-44-5	Clove, copaiba, cannabis	Limited allergen
37	Musk T (Bicyclononalactone)	116-66-5	Synthetic musk	Limited allergen
38	Butylphenylmethylpropional (Lilial duplicate)	80-54-6	Synthetic	Prohibited
39	Camphor	76-22-2	Camphor tree oil	Limited allergen
41	Carvone	2244-16-8	Caraway, spearmint	Limited allergen
42	Cedrol	77-53-2	Cedarwood oils	Limited allergen
43	Cinnamyl Anthranilate	87-29-6	Synthetic	Limited allergen
44	Cis-3-Hexenyl Salicylate	65405-77-8	Green, leafy scent	Limited allergen
45	Citronellyl Acetate	150-84-5	Geranium, citronella	Limited allergen
46	Citronellyl Nitrile	51566-62-2	Synthetic	Limited allergen
47	Cyclamenaldehyde	103-95-7	Synthetic flowers	Limited allergen
48	Dihydroisojasmon	17283-81-7	Jasmine-like, synthetic	Limited allergen
49	Dimethyl anthranilate	85-91-6	Synthetic, fruity	Limited allergen
50	Ethyl vanillin	121-32-4	Vanilla-like, synthetic	Limited allergen
51	Eugenyl acetate	93-28-7	Clove, cinnamon leaf	Limited allergen
52	Florhydral (3-(4-tert-butylphenyl)-2-methylpropanal)	80-54-6	Synthetic	Limited allergen
53	Geranyl acetate	105-87-3	Rose, citronella	Limited allergen
54	Helional (3-(4-Methylphenyl)propane)	1205-17-0	Synthetic	Limited allergen
55	Hexyl salicylate	6259-76-3	Synthetic floral	Limited allergen
56	Indole	120-72-9	Jasmine, orange blossom	Low allergenicity
57	Isoamyl salicylate	87-20-7	Jasmine-like, synthetic	Limited allergen
58	Isobutylphenyl propional	125-68-8	Synthetic	Limited allergen
59	Isopulegol	89-79-2	Mint oils	Limited allergen
60	Linalyl acetate	115-95-7	Lavender, bergamot	Limited allergen
61	Methyl 2-nonynoate	111-80-8	Synthetic, fruity-green	Limited allergen
62	Methylchavicol (Estragol)	140-67-0	Basil, tarragon	Limited allergen
63	Methyl eugenol	93-15-2	Basil, ylang-ylang	Limited allergen
64	Methyl-iso-eugenol	93-16-3	Synthetic	Limited allergen
65	Methyl salicylate	119-36-8	Wintergreen oil	Limited allergen
66	Methyl-N-methyl anthranilate	85-91-6	Synthetic	Limited allergen
67	Nerol	106-25-2	Rose, lemongrass	Limited allergen
68	Ocimene	13877-91-3	Citrus, mint	Limited allergen
69	p-Mentha-1,4-dien-7-ol	499-75-2	Mint oils	Limited allergen
70	p-Mentha-1,4-dien-7-yl Acetate	67674-46-8	Synthetic	Limited allergen
71	Phenylacetaldehyde	122-78-1	Hyacinth, rose	Limited allergen
72	Phenylacetic acid	103-82-2	Honey, cocoa	Limited allergen
73	Phenylacetonitrile	140-29-4	Synthetic	Limited allergen
74	Phenylpropyl Alcohol	122-97-4	Synthetic floral	Limited allergen
75	Precyclestone B	68155-66-8	Synthetic musk	Limited allergen
76	Rose ketones (Ionones, Damascenones, Damascenones)	Miscellaneous	Synthetic rose oil	Restricted allergen
77	Sclareolide	564-20-5	Clary sage	Limited allergen
78	Terpineol	8000-41-7	Pine, petitgrain	Limited allergen
79	Terpinyl acetate	8007-35-0	Pine oil	Limited allergen
80	Tetrahydro Linalool	18479-58-8	Synthetic	Limited allergen
81	Tetrahydro Myrcenol	18479-57-7	Synthetic	Limited allergen
82	Vanillin	121-33-5	Vanilla beans, synthetic	Limited allergen

* Regulatory status: “”Limited allergen”” = must be declared above the threshold values (0.001% leave-on / 0.01% rinse-off). “”Prohibited”” = no longer permitted, but still included in analytical screening. “