Reliable Allergen Testing for Cosmetics, Fragrance, and Home Products

Fragrance allergens are at the heart of EU cosmetic and chemical regulations. Whether they occur naturally in essential oils or are part of synthetic fragrance blends, their presence needs to be measured and declared with precision. Our laboratory offers advanced detection of 82 regulated fragrance allergens using GC-MS/MS technology. This service ensures you stay compliant with EU Cosmetic Regulation 1223/2009, CLP, and IFRA Standards, while also protecting consumer safety and brand reputation.

Why Allergen Testing Matters

Across Europe, companies must declare allergens on cosmetic and detergent labels when concentrations exceed regulatory thresholds. For cosmetics, this means anything above 0.001% in leave-on products or 0.01% in rinse-off products must be listed.

In the past, only 26 allergens needed disclosure, but the list has now expanded to 82 substances. This change affects everyone in the supply chain — from raw material suppliers to brands and safety assessors — and makes reliable allergen testing more important than ever.

What We Test

The method covers the full spectrum of regulated fragrance allergens, including familiar compounds such as Linalool, Citral, Geraniol, Eugenol, Limonene, Coumarin, Benzyl Benzoate, and Farnesol. These substances are often naturally present in essential oils but also appear in synthetic blends. Because their levels vary by source and batch, testing is the only way to ensure accurate disclosure.

✔ A complete list of all 82 allergens is available upon request.

Technology We Use: GC-MS/MS

Our laboratory relies on Gas Chromatography with Tandem Mass Spectrometry (GC-MS/MS), the gold standard for fragrance allergen detection. This method separates complex fragrance mixtures into their individual components and identifies them with high precision.

What makes GC-MS/MS the preferred choice:

• Detects allergens at very low concentrations (ppm and ppb range).

• Provides quantitative results rather than simple “present/absent” findings.

• Works equally well for raw materials and finished products.

In short, GC-MS/MS allows us to deliver data you can rely on for compliance and formulation decisions.

Applications of the 82 Allergen Test

Our allergen detection service is designed for a wide range of industries. Cosmetic brands use it to prepare their CPSR and product labels. Candle and diffuser makers depend on it for IFRA compliance and safe consumer labelling. Raw material suppliers benefit from verified data when selling essential oils and fragrance blends.

Other relevant applications include:

• Cosmetics: perfumes, lotions, hair care, skincare, makeup.

• Candles and home fragrance: ensuring safe allergen disclosures.

• Detergents and cleaning products: meeting CLP and Detergent Regulation standards.

Benefits for Your Business

Accurate allergen testing offers more than just compliance. It provides independent verification of supplier documentation, ensures your product information is trustworthy, and supports smooth approval by safety assessors.

Key advantages include:

• Verified compliance with EU allergen disclosure rules.

• Essential data for Cosmetic Product Safety Reports (CPSR).

• Support for creating IFRA Conformity Certificates.

• Transparent allergen declarations that build consumer confidence.

How the Process Works

Submitting a sample is simple. Send us a sealed quantity of 10–20 g or ml (depending on the product), and we’ll handle the rest. The sample is analysed by GC-MS/MS, and you receive a detailed report with quantified results for all 82 allergens. Reports are delivered as PDF or Excel files, ready to use for regulatory documentation.

• Turnaround time: 7–10 working days (express analysis available).

• Reporting: clear concentration values for every allergen.

• Use case: directly applicable to CPSR, IFRA, PCN, and CLP compliance.

Who Should Use This Test

This service is essential for:

• Cosmetic brands launching products in the EU/EEA.

• Fragrance oil suppliers validating natural or synthetic blends.

• Candle and diffuser makers needing allergen sheets and IFRA compliance.

• Safety assessors and toxicologists who require verified analytical data.

Why Testing Makes a Difference

Consider lavender essential oil. It naturally contains compounds such as linalool and linalyl acetate, but concentrations vary significantly depending on harvest and origin. Without testing, it’s impossible to know whether the allergen levels exceed regulatory thresholds.

Synthetic fragrance blends can be even more complex, often containing multiple allergens in varying proportions. GC-MS/MS testing ensures these details are measured and reported accurately, protecting both compliance and consumer safety.

Beyond Testing

We also provide follow-up services to help you act on the results:

• Preparation of IFRA Certificates.

• Creation of allergen declaration sheets in multiple EU languages.

• Toxicological pre-screening for formulations.

• Full compliance packages including CPSR, PCN, UFI, and CLP support.

82 Fragrance Allergens (EU, Annex III)

Here’s the full list of the 82 fragrance allergens that are included in the new expanded EU Cosmetic Regulation Annex III.

#	INCI Name	CAS Number	Typical Source	Regulatory Status*
1	Alpha-Isomethyl Ionone	127-51-5	Synthetic violet-like fragrance	Restricted allergen
2	Amyl Cinnamal	122-40-7	Jasmine, synthetic	Restricted allergen
3	Amylcinnamyl Alcohol	101-85-9	Jasmine, synthetic	Restricted allergen
4	Anisyl Alcohol	105-13-5	Anise oil, synthetic	Restricted allergen
5	Benzyl Alcohol	100-51-6	Balsams, jasmine	Restricted allergen
6	Benzyl Benzoate	120-51-4	Balsams, essential oils	Restricted allergen
7	Benzyl Cinnamate	103-41-3	Balsam of Peru	Restricted allergen
8	Benzyl Salicylate	118-58-1	Ylang-ylang, synthetic	Restricted allergen
9	Cinnamal	104-55-2	Cinnamon oil	Restricted allergen
10	Cinnamyl Alcohol	104-54-1	Cinnamon leaves	Restricted allergen
11	Citral	5392-40-5	Lemongrass, citrus oils	Restricted allergen
12	Citronellol	106-22-9	Rose, geranium	Restricted allergen
13	Coumarin	91-64-5	Tonka beans	Restricted allergen
14	Eugenol	97-53-0	Clove oil	Restricted allergen
15	Farnesol	4602-84-0	Rose oil, orange blossom	Restricted allergen
16	Geraniol	106-24-1	Rose, palmarosa, citronella	Restricted allergen
17	Hexyl Cinnamal	101-86-0	Jasmine-like scent	Restricted allergen
18	Hydroxycitronellal	107-75-5	Lily of the valley, synthetic	Restricted allergen
19	Hydroxyisohexyl 3-Cyclohexene Carboxaldehyde (Lyral)	31906-04-4	Synthetic	Banned (still monitored)
20	Isoeugenol	97-54-1	Ylang-ylang, clove	Restricted allergen
21	Limonene	5989-27-5	Citrus peel oils	Restricted allergen
22	Linalool	78-70-6	Lavender, coriander	Restricted allergen
23	Methyl 2-Octynoate (Methyl Heptine Carbonate)	111-12-6	Synthetic, fruity-green	Restricted allergen
24	Evernia Prunastri Extract (Oak Moss)	90028-68-5	Lichen extract	Restricted allergen
25	Evernia Furfuracea Extract (Tree Moss)	90028-67-4	Lichen extract	Restricted allergen
26	2-(4-tert-Butylbenzyl)propionaldehyde (Lilial)	80-54-6	Synthetic floral	Banned (still monitored)
27	Allyl Heptanoate	142-19-8	Pineapple-like, synthetic	Restricted allergen
28	Allyl Hexanoate	123-68-2	Pineapple, synthetic	Restricted allergen
29	Allyl Phenoxyacetate	7493-74-5	Sweet floral, synthetic	Restricted allergen
30	Allyl 3-Cyclohexylpropionate	2705-87-5	Fruity, synthetic	Restricted allergen
31	Anethole	4180-23-8	Anise, fennel	Restricted allergen
32	Benzaldehyde	100-52-7	Almonds, cinnamon oil	Restricted allergen
33	Benzyl Acetate	140-11-4	Jasmine, ylang-ylang	Restricted allergen
34	Benzyl Formate	104-57-4	Fruity, balsamic	Restricted allergen
35	Benzyl Isoeugenol	120-11-6	Synthetic	Restricted allergen
36	beta-Caryophyllene	87-44-5	Clove, copaiba, cannabis	Restricted allergen
37	Musk T (Bicyclononalactone)	116-66-5	Synthetic musk	Restricted allergen
38	Butylphenyl Methylpropional (Lilial duplicate)	80-54-6	Synthetic	Banned
39	Camphor	76-22-2	Camphor tree oil	Restricted allergen
40	Cananga Oil	68606-83-7	Ylang-ylang type	Restricted allergen
41	Carvone	2244-16-8	Caraway, spearmint	Restricted allergen
42	Cedrol	77-53-2	Cedarwood oils	Restricted allergen
43	Cinnamyl Anthranilate	87-29-6	Synthetic	Restricted allergen
44	Cis-3-Hexenyl Salicylate	65405-77-8	Green, leafy scent	Restricted allergen
45	Citronellyl Acetate	150-84-5	Geranium, citronella	Restricted allergen
46	Citronellyl Nitrile	51566-62-2	Synthetic	Restricted allergen
47	Cyclamen Aldehyde	103-95-7	Synthetic floral	Restricted allergen
48	Dihydroisojasmone	17283-81-7	Jasmine-like, synthetic	Restricted allergen
49	Dimethyl Anthranilate	85-91-6	Synthetic, fruity	Restricted allergen
50	Ethyl Vanillin	121-32-4	Vanilla-like, synthetic	Restricted allergen
51	Eugenyl Acetate	93-28-7	Clove, cinnamon leaf	Restricted allergen
52	Florhydral (3-(4-tert-Butylphenyl)-2-methylpropanal)	80-54-6	Synthetic	Restricted allergen
53	Geranyl Acetate	105-87-3	Rose, citronella	Restricted allergen
54	Helional (3-(4-Methylphenyl)propanal)	1205-17-0	Synthetic	Restricted allergen
55	Hexyl Salicylate	6259-76-3	Synthetic floral	Restricted allergen
56	Indole	120-72-9	Jasmine, orange blossom	Restricted allergen
57	Isoamyl Salicylate	87-20-7	Jasmine-like, synthetic	Restricted allergen
58	Isobutylphenyl Propional	125-68-8	Synthetic	Restricted allergen
59	Isopulegol	89-79-2	Mint oils	Restricted allergen
60	Linalyl Acetate	115-95-7	Lavender, bergamot	Restricted allergen
61	Methyl 2-Nonynoate	111-80-8	Synthetic, fruity-green	Restricted allergen
62	Methyl Chavicol (Estragole)	140-67-0	Basil, tarragon	Restricted allergen
63	Methyl Eugenol	93-15-2	Basil, ylang-ylang	Restricted allergen
64	Methyl Iso-Eugenol	93-16-3	Synthetic	Restricted allergen
65	Methyl Salicylate	119-36-8	Wintergreen oil	Restricted allergen
66	Methyl-N-methyl Anthranilate	85-91-6	Synthetic	Restricted allergen
67	Nerol	106-25-2	Rose, lemongrass	Restricted allergen
68	Ocimene	13877-91-3	Citrus, mint oils	Restricted allergen
69	p-Mentha-1,4-dien-7-ol	499-75-2	Mint oils	Restricted allergen
70	p-Mentha-1,4-dien-7-yl Acetate	67674-46-8	Synthetic	Restricted allergen
71	Phenyl Acetaldehyde	122-78-1	Hyacinth, rose	Restricted allergen
72	Phenylacetic Acid	103-82-2	Honey, cocoa	Restricted allergen
73	Phenylacetonitrile	140-29-4	Synthetic	Restricted allergen
74	Phenylpropyl Alcohol	122-97-4	Synthetic floral	Restricted allergen
75	Precyclemone B	68155-66-8	Synthetic musk	Restricted allergen
76	Rose Ketones (Ionones, Damascones, Damascenones)	Various	Rose oil, synthetic	Restricted allergen
77	Sclareolide	564-20-5	Clary sage	Restricted allergen
78	Terpineol	8000-41-7	Pine, petitgrain	Restricted allergen
79	Terpinyl Acetate	8007-35-0	Pine oils	Restricted allergen
80	Tetrahydro Linalool	18479-58-8	Synthetic	Restricted allergen
81	Tetrahydro Myrcenol	18479-57-7	Synthetic	Restricted allergen
82	Vanillin	121-33-5	Vanilla pods, synthetic	Restricted allergen

* Regulatory Status: “Restricted allergen” = must be declared above thresholds (0.001% leave-on / 0.01% rinse-off). “Banned” = no longer permitted but still included in analytical screening.